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Serotonin transporter gene promoter methylation in peripheral cells in healthy adults: Neural correlates and tissue specificity.
- Source :
-
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology [Eur Neuropsychopharmacol] 2017 Oct; Vol. 27 (10), pp. 1032-1041. Date of Electronic Publication: 2017 Jul 31. - Publication Year :
- 2017
-
Abstract
- Early adversity can influence gene expression via epigenetic mechanisms, including DNA methylation. Peripheral tissues are essential in psychiatric epigenetics, as methylation generally cannot be assessed in the living human brain. Several magnetic resonance imaging (MRI) studies show associations of peripheral serotonin transporter gene (SLC6A4) methylation with function and/or structure of frontal-limbic circuits and brain's resting-state. Commonly used samples are derived from blood, saliva or buccal cells. However, little is known regarding which peripheral tissue is most strongly associated with human brain processes. The aim of the current study was to compare the extent of the association between peripheral SLC6A4 promoter methylation and frontal-limbic function, structure and resting-state in healthy individuals across peripheral tissues. Forty healthy prospectively-followed adults underwent anatomical, resting-state and functional MRI. Saliva-, blood- and buccal-derived DNA methylation was assessed by pyrosequencing. Blood-derived SLC6A4 methylation was positively associated with superior frontal gray matter (GM) volume and with right lateral parietal area (RLP)-frontal pole regional resting-state functional connectivity (rsFC). Saliva-derived SLC6A4 methylation was positively associated with superior frontal GM volume. Buccal-derived SLC6A4 methylation was positively associated with superior and inferior frontal and anterior cingulate cortical (ACC) GM volumes, and with RLP-ACC, frontal pole and medial prefrontal regional rsFC. Current results confirmed the relevance of peripheral methylation for frontal-limbic processes in humans. Buccal cells may be the most sensitive cell type when studying SLC6A4 promoter methylation and its associated risk for neural vulnerability and resilience for psychopathologies in which serotonin is implicated. These data should be further validated in clinical populations.<br /> (Copyright © 2017 Elsevier B.V. and ECNP. All rights reserved.)
- Subjects :
- Adult
Brain Mapping
Cheek
Epithelial Cells metabolism
Female
Follow-Up Studies
Frontal Lobe diagnostic imaging
Gray Matter diagnostic imaging
Gray Matter physiology
Humans
Limbic System diagnostic imaging
Longitudinal Studies
Magnetic Resonance Imaging
Male
Parietal Lobe diagnostic imaging
Parietal Lobe physiology
Prospective Studies
Rest
Saliva metabolism
DNA Methylation
Frontal Lobe physiology
Limbic System physiology
Promoter Regions, Genetic
Serotonin Plasma Membrane Transport Proteins genetics
Serotonin Plasma Membrane Transport Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7862
- Volume :
- 27
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 28774705
- Full Text :
- https://doi.org/10.1016/j.euroneuro.2017.07.005