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Polyadenylylation of an mRNA precursor occurs independently of transcription by RNA polymerase II in vivo.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1986 Nov; Vol. 83 (22), pp. 8555-9. - Publication Year :
- 1986
-
Abstract
- Most eukaryotic messenger RNAs are transcribed as precursor molecules that must be processed by capping, splicing, 3' cleavage, and polyadenylylation to yield mature mRNAs. An important, unresolved issue is whether any of these reactions are linked either to transcription by RNA polymerase II or to each other. To address one aspect of this question, we constructed a chimeric gene containing an RNA polymerase III promoter (the adenovirus VAI promoter) fused to the body and 3'-flanking sequences of a protein-coding gene (the herpesvirus tk gene). Here we show that this hybrid gene was transcribed from the RNA polymerase III promoter following transfection of human 293 cells and that the transcripts produced were stable and efficiently transported to the cytoplasm. Although a significant proportion of the transcripts were prematurely terminated at specific sites within the gene, a high percentage of the full-length RNA was accurately cleaved and polyadenylylated. These results demonstrate that cleavage and polyadenylylation of mRNA precursors are not obligatorily coupled to transcription by RNA polymerase II in vivo.
- Subjects :
- Endonucleases pharmacology
Humans
Nucleotide Mapping
Promoter Regions, Genetic
RNA Polymerase III pharmacology
RNA Precursors
Single-Strand Specific DNA and RNA Endonucleases
Thymidine Kinase genetics
Nucleic Acid Precursors metabolism
Poly A metabolism
RNA Polymerase II pharmacology
RNA, Messenger metabolism
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 83
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 2877459
- Full Text :
- https://doi.org/10.1073/pnas.83.22.8555