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Activity of rosuvastatin in tachyzoites of Toxoplasma gondii (RH strain) in HeLa cells.
- Source :
-
Experimental parasitology [Exp Parasitol] 2017 Oct; Vol. 181, pp. 75-81. Date of Electronic Publication: 2017 Aug 01. - Publication Year :
- 2017
-
Abstract
- Due to the toxicity of conventional medication in toxoplasmosis, some drugs are being studied for treating this infection, such as statins, especially rosuvastatin compound, which is efficient in inhibiting the initial isoprenoid biosynthesis processes in humans and the parasite. The goal of this study was to assess the activity of rosuvastatin in HeLa cells infected with the RH strain of T. gondii. In the experiment, HeLa cells (1 × 10 <superscript>5</superscript> ) were infected with tachyzoites of T. gondii (5 × 10 <superscript>5</superscript> ). After the experimental infection, we assessed the number of infected cells and the amount of intracellular tachyzoites. In addition, culture supernatants were collected to determine the amount of cytokines by cytometric bead array. We observed that there was no cytotoxicity in the concentrations tested in this cell line. The effect of rosuvastatin showed a significant reduction in both the number of infected cells and the proliferation index of the intracellular parasite, when compared with the conventional treatment combining sulfadiazine and pyrimethamine for toxoplasmosis. There were also reduced levels of cytokines IL-6 and IL-17. Therefore, it was concluded that rosuvastatin exhibited antiproliferative activity. The data presented are significant to promote further studies and the search for alternative treatment for toxoplasmosis.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Analysis of Variance
Antiprotozoal Agents pharmacology
Culture Media
HeLa Cells drug effects
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors toxicity
Interleukin-17 metabolism
Interleukin-6 metabolism
Pyrimethamine pharmacology
Rosuvastatin Calcium toxicity
Sulfadiazine pharmacology
Toxoplasma immunology
HeLa Cells parasitology
Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology
Rosuvastatin Calcium pharmacology
Toxoplasma drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 181
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 28774497
- Full Text :
- https://doi.org/10.1016/j.exppara.2017.07.009