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Estimation of the sensitivity and specificity of two serum ELISAs and one fecal qPCR for diagnosis of paratuberculosis in sub-clinically infected young-adult French sheep using latent class Bayesian modeling.
- Source :
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BMC veterinary research [BMC Vet Res] 2017 Aug 03; Vol. 13 (1), pp. 230. Date of Electronic Publication: 2017 Aug 03. - Publication Year :
- 2017
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Abstract
- Background: The objective was to evaluate the diagnostic accuracy of two serum ELISAs and one quantitative PCR on feces for the diagnosis of paratuberculosis in sub-clinically infected young-adult sheep. A cross-sectional study was performed to collect 1197 individual blood and fecal samples from 2- to 3-year-old sub-clinically infected ewes in 14 closed meat sheep flocks in France. Fecal excretion was determined using qPCR based on IS900 sequence detection, and serology was performed on serum samples using two commercial ELISAs. Data were analyzed in a 3-test multiple-population Bayesian latent class model accounting for potential dependence between the three tests fitted in OpenBUGS. Separate analyses were performed according to whether doubtful ELISA results were handled as positive or negative and based on two thresholds for fecal qPCR (Ct ≤ 42 or Ct ≤ 40).<br />Results: The best fit to the data was provided by accounting for a pairwise dependence between the two ELISAs on sensitivity and pairwise dependence between the three tests on specificity. Under this model, the estimated ELISA sensitivities were 17.4% (95% PCI: 10.6 - 25.9) and 17.9% (95% PCI 11.4 - 25.6), with estimated specificities of 94.8% (95% PCI: 93.1 - 96.3) and 94.0% (95% PCI: 92.2 - 95.7). Fecal qPCR demonstrated significantly higher sensitivity (47.5%; 95% PCI: 29.3 - 69.9) and specificity (99.0%; 95% PCI: 97.9 - 99.9) than the ELISAs. Assumptions regarding doubtful ELISA results and qPCR thresholds had only a slight impact on test accuracy estimates. Models not accounting for pairwise dependence between ELISA and fecal qPCR results yielded higher sensitivity and specificity estimates but always provided a worse fit to the data.<br />Conclusions: Although the overall sensitivity of serum ELISAs and fecal qPCR remains low, the higher diagnostic performances of fecal qPCR make it more suitable for paratuberculosis diagnosis in sub-clinically infected sheep. Our results also illustrate that all dependence structures should be investigated when evaluating diagnostic test accuracy and selection based on a rigorous statistical approach.
- Subjects :
- Animals
Bayes Theorem
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay methods
Feces microbiology
Female
France
Real-Time Polymerase Chain Reaction methods
Sensitivity and Specificity
Sheep
Enzyme-Linked Immunosorbent Assay veterinary
Mycobacterium avium subsp. paratuberculosis isolation & purification
Paratuberculosis diagnosis
Real-Time Polymerase Chain Reaction veterinary
Sheep Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1746-6148
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 28774299
- Full Text :
- https://doi.org/10.1186/s12917-017-1145-x