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M kit : A cell migration assay based on microfluidic device and smartphone.

Authors :: Yang K
Wu J
Peretz-Soroka H
Zhu L
Li Z
Sang Y
Hipolito J
Zhang M
Santos S
Hillier C
de Faria RL
Liu Y
Lin F
Source :: Biosensors & bioelectronics [Biosens Bioelectron] 2018 Jan 15; Vol. 99, pp. 259-267. Date of Electronic Publication: 2017 Jul 27.
Publication Year :: 2018
Abstract: Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS <superscript>2</superscript> technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS <superscript>2</superscript> technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS <superscript>2</superscript> -based cell functional assay for testing cell migration (the M <subscript>kit</subscript> ). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M <subscript>kit</subscript> can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M <subscript>kit</subscript> with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M <subscript>kit</subscript> . In addition to research applications, we demonstrated the effective use of the M <subscript>kit</subscript> for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M <subscript>kit</subscript> provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)