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Antioxidant and Osmoprotecting Activity of Taurine in Dry Eye Models.
- Source :
-
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics [J Ocul Pharmacol Ther] 2018 Jan/Feb; Vol. 34 (1-2), pp. 188-194. Date of Electronic Publication: 2017 Aug 03. - Publication Year :
- 2018
-
Abstract
- Purpose: To assess the protective effects of ophthalmic formulations based on taurine (TAU) and sodium hyaluronate (SH) in ocular surface.<br />Methods: Rabbit corneal epithelial cells [Statens Seruminstitut Rabbit Cornea (SIRC)] were subjected to oxidative stress (1 mM H <subscript>2</subscript> O <subscript>2</subscript> ) and treated with the following formulations: 0.2% SH, 0.4% SH, 0.4% SH +0.5% TAU. Reactive oxygen species (ROS) were evaluated by commercial kit. Dry eye was induced by atropine sulfate and topical treatment was carried out with the following formulations: 0.2% SH, 0.4% SH, 0.4% SH +0.5% TAU. Schirmer's test, tear breakup time (TBUT), and tear osmolarity were evaluated. Furthermore, tear matrix metalloproteinase 9 (MMP-9) expression was assessed by Western blot.<br />Results: TAU significantly (P < 0.05) decreased ROS production in SIRC after oxidative stress. Topical administration of atropine in the rabbit eye significantly (P < 0.05) reduced tear volume and TBUT. Tear osmolarity was also significantly (P < 0.05) modified by atropine treatment. All the altered parameters were significantly (P < 0.05) reversed by 0.5% TAU +0.4% SH treatment; furthermore, this formulation was more effective than SH alone. Moreover, tear levels of MMP-9 were significantly (P < 0.05) lower in the group treated with 0.5% TAU +0.4% SH.<br />Conclusions: Altogether these data suggest that TAU has a relevant antioxidant effect in corneal epithelial cells and prevents the ocular surface damage elicited by atropine. Therefore, our findings suggest that TAU in combination with SH may be useful in clinical practice to manage ocular surface diseases.
- Subjects :
- Administration, Topical
Animals
Antioxidants administration & dosage
Atropine
Cell Line
Cornea drug effects
Cornea metabolism
Dry Eye Syndromes chemically induced
Dry Eye Syndromes metabolism
Epithelial Cells drug effects
Epithelial Cells metabolism
Hyaluronic Acid administration & dosage
Hyaluronic Acid pharmacology
Male
Oxidative Stress drug effects
Rabbits
Reactive Oxygen Species antagonists & inhibitors
Reactive Oxygen Species metabolism
Taurine administration & dosage
Tears drug effects
Tears metabolism
Antioxidants pharmacology
Disease Models, Animal
Dry Eye Syndromes drug therapy
Taurine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7732
- Volume :
- 34
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 28771380
- Full Text :
- https://doi.org/10.1089/jop.2017.0008