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Protection mediated by chemokine CXCL10 in BALB/c mice infected by Leishmania infantum.
- Source :
-
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2017 Aug; Vol. 112 (8), pp. 561-568. - Publication Year :
- 2017
-
Abstract
- Background: Visceral leishmaniasis (VL) caused by Leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. Chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by Leishmania.<br />Objectives: Herein, it was investigated the role of CXCL10 in controlling infection by L. infantum.<br />Methods: RAW 264.7 macrophages were infected with L. infantum in vitro and treated or not with CXCL10 (25, 50 and 100 ng/mL). Parasite load, as well as nitric oxide (NO), IL-4 and IL-10 production were assessed at 24 and 48 h after infection. In vivo, BALB/c mice were infected and treated or not with CXCL10 (5 μg/kg) at one, three and seven days of infection. Parasite load, IFN-g, IL-4, TGF-β and IL-10 were evaluated one, seven and 23 days post treatment.<br />Findings: In vitro, CXCL10 reduced parasitic load, not dependent on NO, and inhibited IL-10 and IL-4 secretion. In vivo, CXCL10 was able to reduce the parasite load in both liver and spleen, four weeks after infection, representing a higher decrease in the number of parasites in these organs, also induced IFN-γ at day 23 after treatment, correlating with the decrease in parasite load, and reduced IL-10 and TGF-β.<br />Main Conclusions: This study suggests a partial protective role of CXCL10 against L. infantum, mediated by IFN-g, not dependent on NO, and with suppression of IL-10 and TGF-β. These data may provide information for the development of new approaches for future therapeutic interventions for VL.
- Subjects :
- Animals
Interferon-gamma analysis
Interleukin-10 biosynthesis
Interleukin-4 biosynthesis
Leishmaniasis, Visceral immunology
Leishmaniasis, Visceral parasitology
Liver parasitology
Liver pathology
Macrophages metabolism
Macrophages parasitology
Male
Mice
Mice, Inbred BALB C
Nitric Oxide biosynthesis
Organ Size
Spleen metabolism
Spleen parasitology
Spleen pathology
Time Factors
Transforming Growth Factor beta analysis
Chemokine CXCL10 therapeutic use
Cytokines immunology
Leishmania infantum
Leishmaniasis, Visceral drug therapy
Macrophages drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1678-8060
- Volume :
- 112
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Memorias do Instituto Oswaldo Cruz
- Publication Type :
- Academic Journal
- Accession number :
- 28767981
- Full Text :
- https://doi.org/10.1590/0074-02760160529