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Analysis of SHIP1 expression and activity in Crohn's disease patients.
- Source :
-
PloS one [PLoS One] 2017 Aug 02; Vol. 12 (8), pp. e0182308. Date of Electronic Publication: 2017 Aug 02 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- Background: SH2 domain containing inositol-5-phosphatase (SHIP1) is an important modulator of innate and adaptive immunity. In mice, loss of SHIP1 provokes severe ileitis resembling Crohn's disease (CD), as a result of deregulated immune responses, altered cytokine production and intestinal fibrosis. Recently, SHIP1 activity was shown to be correlated to the presence of a CD-associated single nucleotide polymorphism in ATG16L1. Here, we studied SHIP1 activity and expression in an adult cohort of CD patients.<br />Methods: SHIP1 activity, protein and mRNA expression in peripheral blood mononuclear cells from CD patients in clinical remission were determined by Malachite green assay, Western blotting and qRT-PCR respectively. Genomic DNA was genotyped for ATG16L1 rs2241880.<br />Results: SHIP1 protein levels are profoundly diminished in a subset of patients; however, SHIP1 activity and expression are not correlated to ATG16L1 SNP status in this adult cohort.<br />Conclusions: Aberrant SHIP1 activity can contribute to disease in a subset of adult CD patients, and warrants further investigation.
- Subjects :
- Adult
Cell Line
Cohort Studies
Crohn Disease metabolism
Female
Gene Expression Regulation
Genetic Association Studies
Genetic Predisposition to Disease
Humans
Male
Middle Aged
Young Adult
Autophagy-Related Proteins genetics
Crohn Disease genetics
Down-Regulation
Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases genetics
Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases metabolism
Polymorphism, Single Nucleotide
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 28767696
- Full Text :
- https://doi.org/10.1371/journal.pone.0182308