An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay.

An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0-3.0μgkg ^-1 and 4.0-10.0μgkg ^-1 , respectively. The average recoveries of MQCA that was spiked into samples at concentrations of 4.0-100μgkg ^-1 were 80.1-87.7%, with relative standard deviations of less than 8.5%. Using the GICA method, the limits of detection for MQCA in different samples were 10μgkg ^-1 and 50μgkg ^-1 . No false negatives or false positives were observed.
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