A polypeptide-mediated synthesis of green fluorescent gold nanoclusters for Fe 3+ sensing and bioimaging.

Authors :: Li H
Huang H
Feng JJ
Luo X
Fang KM
Wang ZG
Wang AJ
Source :: Journal of colloid and interface science [J Colloid Interface Sci] 2017 Nov 15; Vol. 506, pp. 386-392. Date of Electronic Publication: 2017 Jul 18.
Publication Year :: 2017
Abstract: In this paper, growth hormone releasing peptide-6 (GHRP-6) is a bioactive polypeptide and acts as the reducing agent and capping ligand for synthesis of bright green fluorescent gold nanoclusters (GHRP-6-Au NCs) by a simple and environmental-friendly aqueous method, with the assistance of NaBr as the fluorescent sensitizer. The obtained GHRP-6-Au NCs had high fluorescent quantum yield (10.7%), and the fluorescence was strongly quenched by the existence of trace Fe <superscript>3+</superscript> . Thus, a new and highly sensitive sensor for the assay of Fe <superscript>3+</superscript> was constructed based on the analyte-induced fluorescent quenching mechanism. The sensor had a low detection limit of 1.4µM (S/N=3) and a wide linear range of 2-1000µM. Besides, GHRP-6-Au NCs exhibited low cytotoxicity and high biocompatibility for cell imaging.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)

Subjects :: Cell Survival
Chromaffin Cells chemistry
HeLa Cells
Humans
Limit of Detection
Optical Imaging
Particle Size
Sensitivity and Specificity
Spectrometry, Fluorescence
Surface Properties
Fluorescent Dyes chemistry
Gold chemistry
Iron analysis
Metal Nanoparticles chemistry
Oligopeptides chemistry
Peptides chemistry

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