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The permeability characteristics and interaction of main components from Si-Ni-San in a MDCK epithelial cell monolayer model.
- Source :
-
Xenobiotica; the fate of foreign compounds in biological systems [Xenobiotica] 2021 Feb; Vol. 51 (2), pp. 239-248. Date of Electronic Publication: 2020 Dec 02. - Publication Year :
- 2021
-
Abstract
- 1. Si-Ni-San (SNS) possesses extensive therapeutic effects, however, the extent to which main components are absorbed and the mechanisms involved are controversial. 2. In this study, MDCK cell model was used to determine the permeability characteristics and interaction between the major components of Si-Ni-San, including saikosaponin a, paeoniflorin, naringin and glycyrrhizic acid. 3. The transport of the major components was concentration-dependent in both directions. Moreover, the transport of paeoniflorin, naringin and glycyrrhizic acid was significantly reduced at 4 °C or in the presence of NaN3. Additionally, the efflux of paeoniflorin and naringin were apparently reduced in the presence of P-gp inhibitor verapamil. The transport of glycyrrhizic acid was clearly inhibited by the inhibitors of MRP2, indicating that MRP2 may be involved in the transport of glycyrrhizic acid. However, the results indicated that saikosaponin a was absorbed mainly by passive diffusion. Furthermore, the combined incubation of four major components had a powerful sorbefacient effect than a single drug used alone which may be regulated by tight junctions. 4. Taken together, our study provides useful information for pharmacological applications of Si-Ni-San and offers new insights into this ancient decoction for further researches, especially in drug synergism.
- Subjects :
- Animals
Biological Transport
Dogs
Flavanones metabolism
Glucosides metabolism
Glycyrrhizic Acid metabolism
Humans
Madin Darby Canine Kidney Cells
Models, Biological
Monoterpenes metabolism
Oleanolic Acid analogs & derivatives
Oleanolic Acid metabolism
Permeability
Saponins metabolism
Verapamil metabolism
Drugs, Chinese Herbal metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1366-5928
- Volume :
- 51
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Xenobiotica; the fate of foreign compounds in biological systems
- Publication Type :
- Academic Journal
- Accession number :
- 28745128
- Full Text :
- https://doi.org/10.1080/00498254.2017.1359433