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Real-time reverse transcription PCR-based sequencing-independent pathotyping of Eurasian avian influenza A viruses of subtype H7.
- Source :
-
Virology journal [Virol J] 2017 Jul 24; Vol. 14 (1), pp. 137. Date of Electronic Publication: 2017 Jul 24. - Publication Year :
- 2017
-
Abstract
- Low pathogenic avian influenza viruses (LPAIV) of the subtypes H5 and H7 are known to give rise to highly pathogenic (HP) phenotypes by spontaneous insertional mutations which convert a monobasic trypsin-sensitive endoproteolytical cleavage site (CS) within the hemagglutinin (HA) protein into a polybasic subtilisin-sensitive one. Sporadic outbreaks of notifiable LPAIV H7 infections are continuously recorded in Europe and in Asia, and some lineages showed zoonotic transmission. De novo generation of HPAIV H7 from LPAIV precursors has been reported several times over the past decade. Rapid differentiation between LP and HP H7 virus strains is required as a prerequisite to emplace appropriate control measures. Here, reverse transcription real-time PCR assays (RT-qPCR) were developed and evaluated that allow LP and HP pathotype identification and distinction by probe-assisted detection of the HACS. These new RT-qPCRs allow a sensitive and highly specific pathotype identification of Eurasian subtype H7 AIV in allantoic fluids as well as in diagnostic field samples. RT-qPCR assisted pathotyping presents a rapid and sensitive alternative to pathotyping by animal inoculation or nucleotide sequencing.
- Subjects :
- Animals
Asia
Birds
Europe
Influenza A virus isolation & purification
Influenza A virus pathogenicity
Sensitivity and Specificity
Genotype
Genotyping Techniques methods
Influenza A virus classification
Influenza A virus genetics
Influenza in Birds virology
Real-Time Polymerase Chain Reaction methods
Reverse Transcriptase Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1743-422X
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology journal
- Publication Type :
- Academic Journal
- Accession number :
- 28738896
- Full Text :
- https://doi.org/10.1186/s12985-017-0808-3