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Phytaspase-loaded, Mn-doped ZnS quantum dots when embedded into chitosan nanoparticles leads to improved chemotherapy of HeLa cells using in cisplatin.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2017 Oct; Vol. 39 (10), pp. 1591-1598. Date of Electronic Publication: 2017 Jul 20. - Publication Year :
- 2017
-
Abstract
- Objectives: To investigate the potential of recombinant phytaspase loaded manganese (Mn) doped zinc sulphide (ZnS) quantum dots embedded chitosan nanoparticles for augmenting cisplatin induced chemotherapy of HeLa cells.<br />Results: The recombinant phytaspase was cloned into bacterial expression vector PGEX-4T-2. The expressed and purified recombinant plant phytaspase protein from Escherichia coli BL21 was immobilized onto the cationic nanocomposite. Confocal microscopy elucidated the delivery of these luminescent nanocomposites inside cervical cancer HeLa cells. A 50% reduction in the viability of HeLa cells was achieved only in the case of phytaspase-nanocomposites-cisplatin combination at a dose of phytaspase (42 nM), nanocomposites (56.3 μg/ml) and cisplatin (0.44 μg/ml).<br />Conclusion: Luminescent cationic nanocomposites were developed for intracellular delivery of recombinant phytaspase, which due to its caspase-like activity assisted in substantiating the chemotherapeutic activity of apoptosis inducing drug-cisplatin.
- Subjects :
- Cell Proliferation drug effects
Cell Survival drug effects
Gene Expression
HeLa Cells
Humans
Luminescence
Microscopy, Confocal
Microscopy, Electron, Transmission
Nanoparticles
Sulfides chemistry
X-Ray Diffraction
Zinc Compounds chemistry
Chitosan chemistry
Cisplatin pharmacology
Quantum Dots chemistry
Serine Proteases chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 39
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 28730426
- Full Text :
- https://doi.org/10.1007/s10529-017-2395-1