Back to Search
Start Over
Heterologous Expression and Characterization of a Novel Chitinase (ChiEn1) from Coprinopsis cinerea and its Synergism in the Degradation of Chitin.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2017 Aug 16; Vol. 65 (32), pp. 6943-6956. Date of Electronic Publication: 2017 Aug 01. - Publication Year :
- 2017
-
Abstract
- Chitinase ChiEn1 did not hydrolyze insoluble chitin but showed hydrolysis and transglycosylation activities toward chitin-oligosaccharides. Interestingly, the addition of ChiEn1 increased the amount of reducing sugars released from chitin powder by endochitinase ChiIII by 105.0%, and among the released reducing sugars the amount of (GlcNAc) <subscript>2</subscript> was increased by 149.5%, whereas the amount of GlcNAc was decreased by 10.3%. The percentage of GlcNAc in the products of chitin powder with the combined ChiIII and ChiEn1 was close to that in the products of chitin-oligosaccharides with ChiEn1, rather than that with ChiIII. These results indicate that chitin polymers are first degraded into chitin oligosaccharides by ChiIII and the latter are further degraded to monomers and dimers by ChiEn1, and the synergistic action of ChiEn1 and ChiIII is involved in the efficient degradation of chitin in cell walls during pileus autolysis. The structure modeling explores the molecular base of ChiEn1 action.
- Subjects :
- Agaricales metabolism
Biocatalysis
Chitin biosynthesis
Chitinases chemistry
Chitinases isolation & purification
Fungal Proteins chemistry
Fungal Proteins isolation & purification
Agaricales enzymology
Chitin metabolism
Chitinases genetics
Chitinases metabolism
Fungal Proteins genetics
Fungal Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 65
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28721730
- Full Text :
- https://doi.org/10.1021/acs.jafc.7b02278