Thiophene-based rhodamine as selectivef luorescence probe for Fe(III) and Al(III) in living cells.

The thiophene-modified rhodamine 6G (GYJ) has been synthesized as a novel chemosensor. The sensor has sufficiently high selectivity and sensitivity for the detection of Fe ³⁺ and Al ³⁺ ions (M ³⁺ ) by fluorescence and ultraviolet spectroscopy with a strong ability for anti-interference performance. The binding ratio of M ³⁺ -GYJ complex was determined to be 2:1 according to the Job's plot. The binding constants for Fe ³⁺ and Al ³⁺ were calculated to be 3.91 × 10 ⁸ and 5.26 × 10 ⁸  M ^-2 , respectively. All these unique features made it particularly favorable for cellular imaging applications. The obvious fluorescence microscopy experiments demonstrated that the probes could contribute to the detection of Fe ³⁺ and Al ³⁺ in related cells and biological organs with satisfying resolution. Graphical abstract GYJ has high selectivity and sensitivity for the detection of Fe(III) and Al(III) with the binding ratio of 2:1.