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High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast.
- Source :
-
ELife [Elife] 2017 Jul 17; Vol. 6. Date of Electronic Publication: 2017 Jul 17. - Publication Year :
- 2017
-
Abstract
- In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination.
- Subjects :
- Brain Neoplasms
Colorectal Neoplasms
MutL Protein Homolog 1 deficiency
Neoplastic Syndromes, Hereditary
Saccharomyces cerevisiae physiology
Saccharomyces cerevisiae Proteins
DNA, Fungal genetics
Mitosis radiation effects
Nucleic Acid Heteroduplexes analysis
Recombination, Genetic
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae radiation effects
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 28714850
- Full Text :
- https://doi.org/10.7554/eLife.28069