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Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 Sep; Vol. 89 (1), pp. 13-19. Date of Electronic Publication: 2017 Jun 17. - Publication Year :
- 2017
-
Abstract
- Toxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (G1=94) and with clinical suspicion of toxoplasmosis (G2=53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated. The results of the serological methods showed concordance between the ELISA and ELFA methods even though ELFA identified more positive cases than ELISA. Molecular methods were discrepant with cPCR using B22/23 primers having greater sensitivity and lower specificity compared to the other molecular methods.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Adolescent
Adult
Brazil
Enzyme-Linked Immunosorbent Assay methods
Female
Humans
Polymerase Chain Reaction methods
Predictive Value of Tests
Pregnancy
Retrospective Studies
Sensitivity and Specificity
United States
United States Public Health Service
Young Adult
Molecular Diagnostic Techniques methods
Pregnancy Complications, Infectious diagnosis
Serologic Tests methods
Toxoplasmosis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 89
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 28689893
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2017.06.004