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A rapid method for purification of arylsulphatase A from rabbit uterus.

Authors :
Fioretti E
Baldoni E
Bolognani L
Vitaioli L
Source :
Biochemistry international [Biochem Int] 1986 Jan; Vol. 12 (1), pp. 1-9.
Publication Year :
1986

Abstract

The arylsulphatase A from rabbit uterus has been purified with a rapid method using Fast Protein Liquid Chromatography (FPLC). The enzyme is an acid glycoprotein with an apparent molecular weight of 110,000. The enzymatic activity is competitively inhibited by various phosphoesters and various ions such as SO4(2-), PO4(3-) and P2O7(4-).

Details

Language :
English
ISSN :
0158-5231
Volume :
12
Issue :
1
Database :
MEDLINE
Journal :
Biochemistry international
Publication Type :
Academic Journal
Accession number :
2868721