Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts.

Authors :: Pei H
Fu HY
Hirai H
Cho DS
O'Brien TD
Dutton J
Verfaillie CM
Hu WS
Source :: Stem cell research [Stem Cell Res] 2017 May; Vol. 21, pp. 132-136. Date of Electronic Publication: 2017 Apr 21.
Publication Year :: 2017
Abstract: We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M <subscript>3</subscript> O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.<br /> (Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)

Subjects :: Animals
CHO Cells
Cricetinae
Cricetulus
Induced Pluripotent Stem Cells cytology
Kruppel-Like Factor 4
Mice
Induced Pluripotent Stem Cells metabolism
Lentivirus
Transcription Factors biosynthesis
Transcription Factors genetics
Transduction, Genetic

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