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Molecular Subtyping of Salmonella enterica Serovar Typhi by Pulsed-Field Gel Electrophoresis and Multiple-Locus Variable-Number Tandem-Repeat Analysis in India: Their Association with Antimicrobial Resistance Profiles.
- Source :
-
Japanese journal of infectious diseases [Jpn J Infect Dis] 2017 Sep 25; Vol. 70 (5), pp. 536-543. Date of Electronic Publication: 2017 Jul 01. - Publication Year :
- 2017
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Abstract
- Molecular subtyping and DNA sequencing-based methods, which are commonly used for discriminating Salmonella enterica serovar Typhi (S. Typhi) isolates, lead to improved molecular epidemiological investigations for prevention and control of typhoid fever. We obtained S. Typhi blood isolates (n = 66) from India during 2007-14 for molecular subtyping by pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) in association with antibiotic resistance profiles. Genotypic diversity was observed more by MLVA (Simpson's index of diversity, D value = 0.997) than PFGE (D value = 0.864). Two prevalent pulsotypes containing nalidixic acid-resistant (NAL <superscript>R</superscript> ) and NAL <superscript>R</superscript> -ciprofloxacin-resistant (CIP <superscript>R</superscript> ) S. Typhi isolates circulated in India. Multidrug-resistant (MDR), NAL <superscript>R</superscript> -CIP <superscript>R</superscript> , and most NAL <superscript>R</superscript> isolates were found to be clonal by PFGE. MLVA could differentiate the clonal isolates. Most of the MDR and NAL <superscript>R-</superscript> CIP <superscript>R</superscript> isolates showed variation in single or double VNTR loci, whereas NAL <superscript>R</superscript> isolates varied in more than 2 loci, reflecting higher genetic diversity among the NAL <superscript>R</superscript> isolates. Of the 6 VNTR loci, TR4,699 (D value = 0.838) and Sal02 (D value = 0.890) loci played important roles as MLVA cluster-supporting alleles. The rapid turnaround time and high-level discriminatory power of MLVA may be useful for tracking and controlling the transmission of S. Typhi isolates during epidemiological investigations.
- Subjects :
- Anti-Bacterial Agents pharmacology
Electrophoresis, Gel, Pulsed-Field
Humans
India
Minisatellite Repeats
Salmonella typhi genetics
Salmonella typhi isolation & purification
Typhoid Fever microbiology
Drug Resistance, Bacterial
Genotype
Molecular Typing
Salmonella typhi classification
Salmonella typhi drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1884-2836
- Volume :
- 70
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Japanese journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 28674312
- Full Text :
- https://doi.org/10.7883/yoken.JJID.2016.478