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An In Vitro Potency Assay for Monitoring the Immunomodulatory Potential of Stromal Cell-Derived Extracellular Vesicles.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Jul 01; Vol. 18 (7). Date of Electronic Publication: 2017 Jul 01. - Publication Year :
- 2017
-
Abstract
- The regenerative and immunomodulatory activity of mesenchymal stromal cells (MSCs) is partially mediated by secreted vesicular factors. Extracellular vesicles (EVs) exocytosed by MSCs are gaining increased attention as prospective non-cellular therapeutics for a variety of diseases. However, the lack of suitable in vitro assays to monitor the therapeutic potential of EVs currently restricts their application in clinical studies. We have evaluated a dual in vitro immunomodulation potency assay that reproducibly reports the inhibitory effect of MSCs on induced T-cell proliferation and the alloantigen-driven mixed leukocyte reaction of pooled peripheral blood mononuclear cells in a dose-dependent manner. Phytohemagglutinin-stimulated T-cell proliferation was inhibited by MSC-derived EVs in a dose-dependent manner comparable to MSCs. In contrast, inhibition of alloantigen-driven mixed leukocyte reaction was only observed for MSCs, but not for EVs. Our results support the application of a cell-based in vitro potency assay for reproducibly determining the immunomodulatory potential of EVs. Validation of this assay can help establish reliable release criteria for EVs for future clinical studies.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Cell-Derived Microparticles immunology
Cell-Derived Microparticles metabolism
Cells, Cultured
Exosomes immunology
Exosomes metabolism
Extracellular Vesicles immunology
Humans
Isoantigens immunology
Lymphocyte Activation immunology
Lymphocyte Culture Test, Mixed
Mesenchymal Stem Cells metabolism
T-Lymphocytes immunology
T-Lymphocytes metabolism
Extracellular Vesicles metabolism
Immunomodulation
Stromal Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28671586
- Full Text :
- https://doi.org/10.3390/ijms18071413