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[Larvicidal activity of recombinant Escherichia coli expressing scorpion neurotoxin AaIT or B.t.i toxin Cyt2Ba against mosquito larvae and formulations for enhancing the effects].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2017 Jun 20; Vol. 37 (6), pp. 750-754. - Publication Year :
- 2017
-
Abstract
- Objective: To assess the larvicidal effects of recombinant Escherichia coli expressing scorpion neurotoxin AaIT or Bacillus thuringiensis subsp israelensis (B.t.i) toxin Cyt2Ba against the second instar larvae of Culex pipiensquinquefasciatus and Aedes albopictus and compare different formulations for their larvicidal effects.<br />Methods: The AaIT- or Cyt2Ba-coding sequences were cloned into pET28a(+) and the recombinant plasmids were transformed into E. coli BL21(DE3). After induction with IPTG, the recombinant proteins expressed by the recombinant E. coli were detected and identified by SDS-PAGE and Western blotting, respectively. The larvicidal activity of the bacterial suspension was tested at different concentrations against mosquitoes. The effective engineered bacteria were prepared into dry powder with different formulations, and their larvicidal activity was tested.<br />Results: AaIT and Cyt2Ba proteins were successfully expressed in E. coli. The recombinant AaIT protein showed no virulence to the mosquito larvae. The suspension of the recombinant E. coli expressing Cyt2Ba protein exhibited a stronger killing effect on Aedes albopictus larvae than on Culex pipiens quinquefasciatus larvae at 48 h (P<0.001) with LC <subscript>50</subscript> of 3.00×10 <superscript>6</superscript> cells/mL and 1.25×10 <superscript>7</superscript> cells/mL, respectively. The dry powder of the engineered bacteria formulated with yeast extract, wheat flour or white pepper powder at the mass ratio of 1:1 showed the strongest killing effect on mosquito larvae (P=0.044), and the formulation with white pepper powder produced a stronger killing effect than formulations with yeast extract or wheat flour (P=0.002).<br />Conclusion: The B.t.i Cyt2Ba protein expressed in E. coli BL21(DE3) shows a good larvicidal activity against mosquitoes, and appropriate formulations of the engineered bacteria can enhance its efficiency in mosquito control.
- Subjects :
- Animals
Bacillus thuringiensis
Bacillus thuringiensis Toxins
Bacterial Proteins pharmacology
Endotoxins pharmacology
Escherichia coli
Hemolysin Proteins pharmacology
Larva drug effects
Recombinant Proteins biosynthesis
Recombinant Proteins pharmacology
Scorpion Venoms pharmacology
Scorpions
Aedes drug effects
Bacterial Proteins biosynthesis
Culex drug effects
Endotoxins biosynthesis
Hemolysin Proteins biosynthesis
Mosquito Control
Scorpion Venoms biosynthesis
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 37
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 28669947