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ANF stimulation of detergent-dispersed particulate guanylate cyclase from bovine adrenal cortex.
- Source :
-
FEBS letters [FEBS Lett] 1986 Jan 06; Vol. 194 (2), pp. 210-4. - Publication Year :
- 1986
-
Abstract
- Particulate guanylate cyclase from bovine adrenal cortex can be stimulated by ANF. A 2-fold stimulation of the enzyme was obtained with 100 nM ANF and a half-maximal stimulation, with a 5 nM dose. The stimulation by ANF persisted for at least 30 min. Various detergents, such as Triton X-100, Lubrol PX, cholate, CHAPS, digitonin and zwittergent, stimulated several-fold the activity of particulate guanylate cyclase. However, only Triton X-100 dispersed particulate guanylate cyclase without affecting its response to ANF. The dose-response curve of ANF stimulation of the particulate and the Triton X-100 dispersed enzyme was similar. The dispersion of a fully responsive guanylate cyclase to ANF will help us to uncover the type of interactions between guanylate cyclase and ANF. It will also be used as a first step for the purification of an ANF-sensitive particulate guanylate cyclase.
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 194
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 2866981
- Full Text :
- https://doi.org/10.1016/0014-5793(86)80086-2