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Proximal clustering between BK and Ca V 1.3 channels promotes functional coupling and BK channel activation at low voltage.
- Source :
-
ELife [Elife] 2017 Jun 30; Vol. 6. Date of Electronic Publication: 2017 Jun 30. - Publication Year :
- 2017
-
Abstract
- Ca <subscript>V</subscript> -channel dependent activation of BK channels is critical for feedback control of both calcium influx and cell excitability. Here we addressed the functional and spatial interaction between BK and Ca <subscript>V</subscript> 1.3 channels, unique Ca <subscript>V</subscript> 1 channels that activate at low voltages. We found that when BK and Ca <subscript>V</subscript> 1.3 channels were co-expressed in the same cell, BK channels started activating near -50 mV, ~30 mV more negative than for activation of co-expressed BK and high-voltage activated Ca <subscript>V</subscript> 2.2 channels. In addition, single-molecule localization microscopy revealed striking clusters of Ca <subscript>V</subscript> 1.3 channels surrounding clusters of BK channels and forming a multi-channel complex both in a heterologous system and in rat hippocampal and sympathetic neurons. We propose that this spatial arrangement allows tight tracking between local BK channel activation and the gating of Ca <subscript>V</subscript> 1.3 channels at quite negative membrane potentials, facilitating the regulation of neuronal excitability at voltages close to the threshold to fire action potentials.
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 28665272
- Full Text :
- https://doi.org/10.7554/eLife.28029