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Hair growth promoting activity of discarded biocomposite keratin extract.
- Source :
-
Journal of biomaterials applications [J Biomater Appl] 2017 Aug; Vol. 32 (2), pp. 230-241. Date of Electronic Publication: 2017 Jun 29. - Publication Year :
- 2017
-
Abstract
- Keratin biomaterial has been used in regenerative medicine owing to its in-vivo and in-vitro biocompatibility. The present study was aimed to investigate the hair growth promoting activity of keratin extract and its mechanism of action. Keratin extract was topically applied on the synchronized depilated dorsal skin of telogenic C57BL/6 mice and promoted hair growth by inducing the anagen phase. The histomorphometric observation indicated significantly increases the number, shaft of hair follicles and deep subcutis area in the keratin extract treated group in contrast to the control group, which was considered an indication of anagen phase induction. Subsequently, the quantitative real-time polymerase chain reaction analysis revealed that fibroblast growth factor-10, vascular endothelial growth factor, insulin-like growth factor-1, β-catenin, and Shh were expressed earlier in the keratin extract-treated group than in the control group. Besides, keratin extract has been observed to be biocompatible when analyzed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 4',6-diamidino-2-phenylindole staining using immortalized human keratinocyte cells, showing more than 90% cell viability. Our study demonstrated that keratin extract stimulating hair follicle growth by inducing the growth phase; anagen in telogenic C57BL/6 mice and thus the topical application of keratin extract may represent a promising biomaterial for the management and applications of hair follicle disorder.
- Subjects :
- Animals
Cell Line
Cell Proliferation drug effects
Fibroblast Growth Factor 10 genetics
Gene Expression Regulation drug effects
Hair metabolism
Hair Follicle drug effects
Hair Follicle growth & development
Hair Follicle metabolism
Humans
Insulin-Like Growth Factor I genetics
Keratinocytes cytology
Keratinocytes drug effects
Keratinocytes metabolism
Keratins administration & dosage
Keratins isolation & purification
Mice, Inbred C57BL
Vascular Endothelial Growth Factor A genetics
beta Catenin genetics
Hair drug effects
Hair growth & development
Keratins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1530-8022
- Volume :
- 32
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biomaterials applications
- Publication Type :
- Academic Journal
- Accession number :
- 28662599
- Full Text :
- https://doi.org/10.1177/0885328217717076