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Transplantation of mouse-induced pluripotent stem cells into the cochlea for the treatment of sensorineural hearing loss.
- Source :
-
Acta oto-laryngologica [Acta Otolaryngol] 2017 Nov; Vol. 137 (11), pp. 1136-1142. Date of Electronic Publication: 2017 Jun 23. - Publication Year :
- 2017
-
Abstract
- Conclusion: Mouse-induced pluripotent stem cells (iPSCs) could differentiate into hair cell-like cells and spiral ganglion-like cells after transplantation into mouse cochleae, but it cannot improve the auditory brain response (ABR) thresholds in short term.<br />Objective: To evaluate the potential of iPSCs for use as a source of transplants for the treatment of sensorineural hearing loss (SNHL).<br />Methods: Establishing SNHL mice model, then injecting the iPSCs or equal volume DMEM basic medium into the cochleae, respectively. Immunofluorescence staining and reverse transcription-polymerase chain reaction (RT-PCR) were used to assess the survival, migration, differentiation of the transplanted iPSCs in cochleae and then recorded the ABR threshold in different time. Hematoxylin-eosin (HE) staining was used to observe the teratoma formation.<br />Results: Four weeks after transplantation, CM-Di1-labeled iPSCs could be found in the modiolus and Rosenthal's canal (RC), and some of them could expressed auditory hair cell markers or spiral ganglion neuron makers in group A, but not found in group B and C. As to the ABR threshold, no significance differences were found between pre- with postoperative in group A or B. In our study, no teratoma was observed in the cochleae.
- Subjects :
- Animals
Cell Differentiation
Cochlea surgery
Hair Cells, Auditory cytology
Hair Cells, Auditory metabolism
Mice, Inbred ICR
Spiral Ganglion cytology
Spiral Ganglion metabolism
Evoked Potentials, Auditory, Brain Stem
Hearing Loss, Sensorineural surgery
Induced Pluripotent Stem Cells transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1651-2251
- Volume :
- 137
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Acta oto-laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 28643534
- Full Text :
- https://doi.org/10.1080/00016489.2017.1342045