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Characterization of Extended-Spectrum β-Lactamase Genes of Shigella flexneri Isolates With Fosfomycin Resistance From Patients in China.
- Source :
-
Annals of laboratory medicine [Ann Lab Med] 2017 Sep; Vol. 37 (5), pp. 415-419. - Publication Year :
- 2017
-
Abstract
- Background: The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flexneri from patients in China.<br />Methods: A total of 263 S. flexneri isolates were collected from 34 hospitals in the Anhui Province of China during September 2012-September 2015 and screened for fosA3, fosA, and fosC2 by PCR amplification and sequencing. The fos-carrying isolates were then screened for β-lactamase genes. The clonal relationships between fosA3-carrying isolates, the transmissibility of fosfomycin resistance, replicon types of plasmids carrying fosfomycin resistance genes and other associated resistance genes were investigated.<br />Results: Twenty-five of the 263 isolates (9.5%) showed resistance to fosfomycin, and 18 (6.8%) were positive for fosA3. None of the isolates was positive for fosA or fosC2. Seventeen of the isolates carrying fosA3 (94%) were CTX-M producers (seven CTX-M-55, five CTX-M-14, and five CTX-M-123), while three (16.7%) were TEM producers (TEM-1).Sixteen (88.9%) fosA3-carrying isolates exhibited multi-drug resistance. The replicon types of the 13 fosA3-carrying plasmids were IncF (n=13), IncHI2 (n=3), IncIl-Ir (n=2), and IncN (n=1).<br />Conclusions: Our results indicated that fosA3 could spread through plasmids in S. flexneri isolates, along with the bla(CTX-M) and bla(TEM), which facilitate its quick dispersal. To the best of our knowledge, this is the first report of CTX-M-123-type ESBLs in S. flexneri isolates from patients in China.<br />Competing Interests: No potential conflicts of interest relevant to this article were reported.<br /> (© The Korean Society for Laboratory Medicine)
- Subjects :
- China
DNA, Bacterial genetics
DNA, Bacterial metabolism
Drug Resistance, Multiple, Bacterial genetics
Dysentery, Bacillary microbiology
Genotype
Microbial Sensitivity Tests
Plasmids genetics
Plasmids metabolism
Polymerase Chain Reaction
Shigella flexneri isolation & purification
Anti-Bacterial Agents pharmacology
Bacterial Proteins genetics
Dysentery, Bacillary diagnosis
Fosfomycin pharmacology
Shigella flexneri drug effects
Shigella flexneri genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2234-3814
- Volume :
- 37
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Annals of laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 28643490
- Full Text :
- https://doi.org/10.3343/alm.2017.37.5.415