Back to Search
Start Over
MaRAP2-4, a waterlogging-responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis.
- Source :
-
Plant biotechnology journal [Plant Biotechnol J] 2018 Jan; Vol. 16 (1), pp. 221-233. Date of Electronic Publication: 2017 Jul 25. - Publication Year :
- 2018
-
Abstract
- As waterlogging and successive events severely influence growth and development of economically important plants, we attempted to characterize the role of a waterlogging-responsive group I (A-6) ethylene response factor (MaRAP2-4) from Mentha arvensis. Waterlogging, ethylene and methyl jasmonate rapidly induced the expression of MaRAP2-4. MaRAP2-4 interacted with multiple cis-elements like dehydration response elements (DRE1/2), anoxia/jasmonic acid response element (JARE) and GCC box showing its involvement in multiple responses. MaRAP2-4 localizes in the nucleus and acts as a transcriptional activator. Truncation and internal deletion identified a 20 amino acids potential transactivation domain (PLPSSVDAKLEAICQSLAIN) in MaRAP2-4. MaRAP2-4 transgenic Arabidopsis showed enhanced waterlogging and subsequent oxidative stress tolerance. Microarray analysis revealed that within up-regulated genes 483, 212 and 132 promoters carry either single or multiple copies of DRE, JARE and GCC cis-element/s, respectively. Within these promoters, a large section belongs to carbohydrate metabolism/transport, including many SWEET transporters. Further analysis showed MaRAP2-4 specifically targets two positions in AtSWEEET10 promoter carrying DRE and/or GCC box that might regulate carbohydrate availability and waterlogging tolerance. These results demonstrate that MaRAP2-4 is a positive regulator of waterlogging tolerance, and as energy-consuming processes such as carbohydrate biosynthesis are reduced under waterlogging-induced hypoxia, sugar transport through SWEETs may be the primary option to make sugar available to the required tissue.<br /> (© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Subjects :
- Acetates metabolism
Arabidopsis genetics
Arabidopsis Proteins genetics
Cyclopentanes metabolism
Gene Expression Regulation, Plant genetics
Gene Expression Regulation, Plant physiology
Oxylipins metabolism
Plants, Genetically Modified genetics
Promoter Regions, Genetic genetics
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Mentha metabolism
Plants, Genetically Modified metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1467-7652
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant biotechnology journal
- Publication Type :
- Academic Journal
- Accession number :
- 28636266
- Full Text :
- https://doi.org/10.1111/pbi.12762