Back to Search
Start Over
The Application of Heptamethine Cyanine Dye DZ-1 and Indocyanine Green for Imaging and Targeting in Xenograft Models of Hepatocellular Carcinoma.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Jun 21; Vol. 18 (6). Date of Electronic Publication: 2017 Jun 21. - Publication Year :
- 2017
-
Abstract
- Near infrared fluorescence (NIRF) imaging has strong potential for widespread use in noninvasive tumor imaging. Indocyanine green (ICG) is the only Food and Drug Administration (FDA) -approved NIRF dye for clinical diagnosis; however, it is unstable and poorly targets tumors. DZ-1 is a novel heptamethine cyanine NIRF dye, suitable for imaging and tumor targeting. Here, we compared the fluorescence intensity and metabolism of DZ-1 and ICG. Additionally, we assayed their specificities and abilities to target tumor cells, using cultured hepatocellular carcinoma (HCC) cell lines, a nude mouse subcutaneous xenograft model of liver cancer, and a rabbit orthotopic transplantation model. We found that DZ-1 accumulates in tumor tissue and specifically recognizes HCC in subcutaneous and orthotopic models. The NIRF intensity of DZ-1 was one order of magnitude stronger than that of ICG, and DZ-1 showed excellent intraoperative tumor targeting in the rabbit model. Importantly, ICG accumulated at tumor sites, as well as in the liver and kidney. Furthermore, DZ-1 analog-gemcitabine conjugate (NIRG) exhibited similar tumor-specific targeting and imaging properties, including inhibition of tumor growth, in HCC patient-derived xenograft (PDX) mice. DZ-1 and NIRG demonstrated superior tumor-targeting specificity, compared to ICG. We show that DZ-1 is an effective molecular probe for specific imaging, targeting, and therapy in HCC.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Animals
Carbocyanines metabolism
Carcinoma, Hepatocellular metabolism
Cell Line, Tumor
Coloring Agents metabolism
Humans
Indocyanine Green metabolism
Liver metabolism
Liver Neoplasms metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Rabbits
Carbocyanines analysis
Carcinoma, Hepatocellular diagnostic imaging
Coloring Agents analysis
Indocyanine Green analysis
Liver diagnostic imaging
Liver Neoplasms diagnostic imaging
Optical Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28635650
- Full Text :
- https://doi.org/10.3390/ijms18061332