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Expression of Jagged1/Notch3 Signaling Pathway and their Relationship with the Tumor Angiogenesis in TNBC.
- Source :
-
Archives of medical research [Arch Med Res] 2017 Feb; Vol. 48 (2), pp. 169-179. - Publication Year :
- 2017
-
Abstract
- Background and Aims: Jagged1/Notch3 signaling pathway plays a key role in angiogenesis of breast cancer, but little is known in TNBC. This study was designed to investigate the expression of Jagged1/Notch3 mRNA and protein in TNBC, analyze their correlations with clinicopathological characteristics and prognosis. Moreover, the interrelationship among Jagged1/Notch3 and VEGF was initially evaluated.<br />Methods: Jagged1/Notch3 mRNA and protein expression levels were determined by Q-RT-PCR and Western blotting. Additionally, Immunohistochemistry for Jagged1/Notch3 was detected by Ventana platform, VEGF and CD34 was performed using the EnVision/HRP technique.<br />Results: mRNA transcriptionof Jagged1/Notch3 was in accord with protein expression. TNBC patients with positive Jagged1 expression had poorer DFS (p = 0.008) and OS (p = 0.004). Jagged1 expression was independent predictors of OS (p = 0.038). The expression of VEGF was positively correlative to MVD (p = 0.018), MVD was significantly associated with Jagged1 (p <0.0001) and Notch3 (p <0.0001). The expression of Jagged1/Notch3 has no correlation with VEGF, only in positive VEGF expression of TNBC patients Jagged1/Notch3 had influence on DFS and OS (p <0.05).<br />Conclusion: Jagged1/Notch3 was -expressed at both the mRNA and protein levels, Jagged1 served as an independent predictor of poor prognosis. We speculate that there is a cross-talk between Jagged1/Notch3 and VEGF in TNBC angiogenesis. Jagged1/Notch3 is expected to be an important signaling pathway for TNBC progression and a potential target for TNBC neovascularization therapy.<br /> (Copyright © 2017 IMSS. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Female
Humans
Jagged-1 Protein genetics
Neovascularization, Pathologic genetics
Prognosis
RNA, Messenger metabolism
Signal Transduction
Triple Negative Breast Neoplasms blood supply
Triple Negative Breast Neoplasms diagnosis
Vascular Endothelial Growth Factor A metabolism
Jagged-1 Protein metabolism
Neovascularization, Pathologic metabolism
Receptor, Notch3 metabolism
Triple Negative Breast Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5487
- Volume :
- 48
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Archives of medical research
- Publication Type :
- Academic Journal
- Accession number :
- 28625320
- Full Text :
- https://doi.org/10.1016/j.arcmed.2017.03.014