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Tumour heterogeneity poses a significant challenge to cancer biomarker research.
- Source :
-
British journal of cancer [Br J Cancer] 2017 Jul 25; Vol. 117 (3), pp. 367-375. Date of Electronic Publication: 2017 Jun 15. - Publication Year :
- 2017
-
Abstract
- Background: The high degree of genomic diversity in cancer represents a challenge for identifying objective prognostic markers. We aimed to examine the extent of tumour heterogeneity and its effect on the evaluation of a selected prognostic marker using prostate cancer as a model.<br />Methods: We assessed Gleason Score (GS), DNA ploidy status and phosphatase and tensin homologue (PTEN) expression in radical prostatectomy specimens (RP) from 304 patients followed for a median of 10 years (interquartile range 6-12). GS was assessed for every tumour-containing block and DNA ploidy for a median of four samples for each RP. In a subgroup of 40 patients we assessed DNA ploidy and PTEN status in every tumour-containing block. In 102 patients assigned to active surveillance (AS), GS and DNA ploidy were studied in needle biopsies.<br />Results: Extensive heterogeneity was observed for GS (89% of the patients) and DNA ploidy (40% of the patients) in the cohort, and DNA ploidy (60% of the patients) and PTEN expression (75% of the patients) in the subgroup. DNA ploidy was a significant prognostic marker when heterogeneity was taken into consideration. In the AS cohort we found heterogeneity in GS (24%) and in DNA ploidy (25%) specimens.<br />Conclusions: Multi-sample analysis should be performed to support clinical treatment decisions.
- Subjects :
- Aged
Biopsy, Needle
Genetic Heterogeneity
Humans
Male
Middle Aged
Neoplasm Grading
Prostate pathology
Prostatectomy
Prostatic Neoplasms chemistry
Prostatic Neoplasms therapy
Tumor Burden
Watchful Waiting
Biomarkers, Tumor
DNA, Neoplasm analysis
Neoplasm Recurrence, Local genetics
PTEN Phosphohydrolase analysis
Ploidies
Prostatic Neoplasms genetics
Prostatic Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-1827
- Volume :
- 117
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- British journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 28618431
- Full Text :
- https://doi.org/10.1038/bjc.2017.171