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High throughput in vitro and in vivo screening of inland waters of Southern California.
- Source :
-
Environmental science. Processes & impacts [Environ Sci Process Impacts] 2017 Sep 20; Vol. 19 (9), pp. 1142-1149. - Publication Year :
- 2017
-
Abstract
- The impact of unmonitored contaminants, also known as contaminants of emerging concern (CECs), on freshwater streams remains largely uncharacterized. Water samples from 31 streams representing urban, agricultural and undeveloped (i.e., open space) land use in Southern California (USA) were analyzed for in vitro and in vivo bioactivity. The extent and magnitude of bioactivity screened using endocrine-responsive cell bioassays and a fish embryo screening assay were low. In contrast, a wider gradient of responses for the aryl hydrocarbon receptor (AhR) assay was observed, which was negatively correlated with a measure of benthic community structure. Both aromatic and non-aromatic CECs were tentatively identified in these samples, but polycyclic aromatic hydrocarbons (PAHs), known AhR agonists in urban environments, were not present at detectable levels. These results suggest that a combination of in vitro and in vivo show potential as screening techniques for biological condition in situ, but that more advanced, comprehensive analytical methods are needed to identify bioactive contaminants.
- Subjects :
- Animals
California
Embryo, Nonmammalian drug effects
HEK293 Cells
Humans
Polycyclic Aromatic Hydrocarbons analysis
Receptors, Aryl Hydrocarbon metabolism
Receptors, Estrogen metabolism
Receptors, Glucocorticoid metabolism
Water Pollutants, Chemical analysis
Zebrafish
Environmental Monitoring methods
High-Throughput Screening Assays methods
Polycyclic Aromatic Hydrocarbons toxicity
Rivers chemistry
Water Pollutants, Chemical toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7895
- Volume :
- 19
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Environmental science. Processes & impacts
- Publication Type :
- Academic Journal
- Accession number :
- 28612856
- Full Text :
- https://doi.org/10.1039/c7em00170c