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Reverse Zymography: Overview and Pitfalls.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1626, pp. 125-132. - Publication Year :
- 2017
-
Abstract
- Reverse zymography is a technique by which protease inhibitor(s) in a sample could be electrophoretically separated in a substrate-impregnated acrylamide gel and their relative abundance could be semi-quantified. The gel after electrophoresis is incubated with a protease when the impregnated substrate and all other proteins of the sample are degraded into small peptides except the inhibitor(s) that show clear bands against a white background. Since reverse zymography cannot distinguish between a protease inhibitor and a protein that is resistant against proteolysis, the results should be confirmed from inhibition of protease activity by solution state assay.
- Subjects :
- Animals
Humans
Muramidase analysis
Muramidase metabolism
Protease Inhibitors metabolism
Proteolysis
Substrate Specificity
Trypsin metabolism
Trypsin Inhibitors analysis
Trypsin Inhibitors metabolism
Electrophoresis, Polyacrylamide Gel methods
Enzyme Assays methods
Peptide Hydrolases metabolism
Protease Inhibitors analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1626
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 28608205
- Full Text :
- https://doi.org/10.1007/978-1-4939-7111-4_11