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Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis.
- Source :
-
Nature cell biology [Nat Cell Biol] 2017 Jul; Vol. 19 (7), pp. 787-798. Date of Electronic Publication: 2017 Jun 12. - Publication Year :
- 2017
-
Abstract
- The endosomal sorting complex required for transport (ESCRT)-III mediates membrane fission in fundamental cellular processes, including cytokinesis. ESCRT-III is thought to form persistent filaments that over time increase their curvature to constrict membranes. Unexpectedly, we found that ESCRT-III at the midbody of human cells rapidly turns over subunits with cytoplasmic pools while gradually forming larger assemblies. ESCRT-III turnover depended on the ATPase VPS4, which accumulated at the midbody simultaneously with ESCRT-III subunits, and was required for assembly of functional ESCRT-III structures. In vitro, the Vps2/Vps24 subunits of ESCRT-III formed side-by-side filaments with Snf7 and inhibited further polymerization, but the growth inhibition was alleviated by the addition of Vps4 and ATP. High-speed atomic force microscopy further revealed highly dynamic arrays of growing and shrinking ESCRT-III spirals in the presence of Vps4. Continuous ESCRT-III remodelling by subunit turnover might facilitate shape adaptions to variable membrane geometries, with broad implications for diverse cellular processes.
- Subjects :
- ATPases Associated with Diverse Cellular Activities
Endosomal Sorting Complexes Required for Transport genetics
Endosomes ultrastructure
HeLa Cells
Humans
Intracellular Membranes ultrastructure
Microscopy, Atomic Force
RNA Interference
Signal Transduction
Time Factors
Transfection
Vacuolar Proton-Translocating ATPases genetics
Cytokinesis
Endosomal Sorting Complexes Required for Transport metabolism
Endosomes enzymology
Intracellular Membranes enzymology
Vacuolar Proton-Translocating ATPases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4679
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nature cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 28604678
- Full Text :
- https://doi.org/10.1038/ncb3559