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[Inhibitory Effects of Sphingosine-1-phosphate Receptor-2 on Vascular Permeability in Mice].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2016 Sep; Vol. 47 (5), pp. 714-717. - Publication Year :
- 2016
-
Abstract
- Objectives: To determine the effect of sphingosine-1-phosphate receptor 2 (S1PR2) on vascular permeability in mice.<br />Methods: Acute lung injury models of mice were constructed with intra-tracheal administration of lipopolysaccharide (LPS) and compared with the controls with intra-tracheal administration of saline. The effect of S1PR2 on vascular permeability was observed by detecting leakage of Evans blue into lung tissues, pulmonary vascular leakage of fluorescein isothiocyanate (FITC)-dextran, and the wet/dry mass ratio of lungs. The effect of vascular endothelial growth factor (VEGF) on vascular endothelial permeability was detected by Miles analysis.<br />Results: LPS injections induced significant Evans blue leakage, FITC-dextran pulmonary vascular leakage and pulmonary edema, which appeared to be more serious in S1PR2-deleted mice compared with those in wild-type mice. LPS enhanced Evans blue leakage associated with VEGF in a dose-dependent way in both S1PR2-deleted mice and wild type mice. But the vascular permeability response in subcutaneous tissues induced by VEGF was higher in S1PR2-deleted mice than that in wild-type mice.<br />Conclusions: S1PR2 is involved in endothelial cell barrier protections, which inhibits vascular permeability.
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 47
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 28598085