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In vitro evaluation of a basic fibroblast growth factor-containing hydrogel toward vocal fold lamina propria scar treatment.
- Source :
-
Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2018 Apr; Vol. 106 (3), pp. 1258-1267. Date of Electronic Publication: 2017 Jun 05. - Publication Year :
- 2018
-
Abstract
- Scarring of the vocal fold lamina propria can lead to debilitating voice disorders that can significantly impair quality of life. The reduced pliability of the scar tissue-which diminishes proper vocal fold vibratory efficiency-results in part from abnormal extracellular matrix (ECM) deposition by vocal fold fibroblasts (VFF) that have taken on a fibrotic phenotype. To address this issue, bioactive materials containing cytokines and/or growth factors may provide a platform to transition fibrotic VFF within the scarred tissue toward an anti-fibrotic phenotype, thereby improving the quality of ECM within the scar tissue. However, for such an approach to be most effective, the acute host response resulting from biomaterial insertion/injection likely also needs to be considered. The goal of the present work was to evaluate the anti-fibrotic and anti-inflammatory capacity of an injectable hydrogel containing tethered basic fibroblast growth factor (bFGF) in the dual context of scar and biomaterial-induced acute inflammation. An in vitro co-culture system was utilized containing both activated, fibrotic VFF and activated, pro-inflammatory macrophages (MΦ) within a 3D poly(ethylene glycol) diacrylate (PEGDA) hydrogel containing tethered bFGF. Following 72 h of culture, alterations in VFF and macrophage phenotype were evaluated relative to mono-culture and co-culture controls. In our co-culture system, bFGF reduced the production of fibrotic markers collagen type I, α smooth muscle actin, and biglycan by activated VFF and promoted wound-healing/anti-inflammatory marker expression in activated MΦ. Cumulatively, these data indicate that bFGF-containing hydrogels warrant further investigation for the treatment of vocal fold lamina propria scar. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1258-1267, 2018.<br /> (© 2017 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Cells, Cultured
Cicatrix pathology
Coculture Techniques
Cytokines biosynthesis
Extracellular Matrix pathology
Fibroblast Growth Factor 2 administration & dosage
Fibroblast Growth Factor 2 therapeutic use
Fibroblasts pathology
Fibrosis drug therapy
Humans
Macrophage Activation drug effects
Mice
RAW 264.7 Cells
Rheology
Swine
Wound Healing drug effects
Cicatrix surgery
Fibroblast Growth Factor 2 pharmacology
Hydrogels
Vocal Cords pathology
Vocal Cords surgery
Subjects
Details
- Language :
- English
- ISSN :
- 1552-4981
- Volume :
- 106
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of biomedical materials research. Part B, Applied biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 28580765
- Full Text :
- https://doi.org/10.1002/jbm.b.33936