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MiR-375 delivered by lipid-coated doxorubicin-calcium carbonate nanoparticles overcomes chemoresistance in hepatocellular carcinoma.
- Source :
-
Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2017 Nov; Vol. 13 (8), pp. 2507-2516. Date of Electronic Publication: 2017 Jun 01. - Publication Year :
- 2017
-
Abstract
- Hepatocellular carcinoma (HCC) is a prevalent and lethal disease that is characterized by drug resistance. Doxorubicin (DOX) is a widely used chemotherapeutic drug and miR-375 has been shown to be a tumor suppressor in HCC. Here, we reported that miR-375 and DOX co-loaded into lipid-coated calcium carbonate nanoparticles (LCC-DOX/miR-375 NPs), enhanced the anti-tumor effects through combination therapy, and were highly effective in reversing drug resistance in HCC. LCC-DOX/miR-375 NPs were prepared by a reverse microemulsions method. In vitro, LCC-DOX/miR-375 NPs exhibited enhanced intracellular accumulation, pH-sensitive DOX release and potent cytotoxicity. In vivo, LCC-DOX/miR-375 NPs showed efficient antitumor effect both in xenograft and primary HCC murine models. Our results showed that the LCC-DOX/miR-375 nanoparticles provide a novel strategy to overcome the drug resistance and promote addictive effect between miR-375 and DOX in HCC.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antibiotics, Antineoplastic therapeutic use
Carcinoma, Hepatocellular drug therapy
Carcinoma, Hepatocellular pathology
Cell Line, Tumor
Doxorubicin therapeutic use
Drug Delivery Systems
Drug Resistance, Neoplasm
Hep G2 Cells
Humans
Liver Neoplasms drug therapy
Liver Neoplasms pathology
Mice
Mice, Nude
MicroRNAs therapeutic use
Antibiotics, Antineoplastic administration & dosage
Calcium Carbonate chemistry
Carcinoma, Hepatocellular therapy
Doxorubicin administration & dosage
Lipids chemistry
Liver Neoplasms therapy
MicroRNAs administration & dosage
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1549-9642
- Volume :
- 13
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 28577837
- Full Text :
- https://doi.org/10.1016/j.nano.2017.05.010