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Alginate Microcapsules Incorporating Hyaluronic Acid Recreate Closer in Vivo Environment for Mesenchymal Stem Cells.
- Source :
-
Molecular pharmaceutics [Mol Pharm] 2017 Jul 03; Vol. 14 (7), pp. 2390-2399. Date of Electronic Publication: 2017 Jun 06. - Publication Year :
- 2017
-
Abstract
- The potential clinical application of alginate cell microencapsulation has advanced enormously during the past decade. However, the 3D environment created by alginate beads does not mimic the natural extracellular matrix surrounding cells in vivo, responsible of cell survival and functionality. As one of the most frequent macromolecules present in the extracellular matrix is hyaluronic acid, we have formed hybrid beads with alginate and hyaluronic acid recreating a closer in vivo cell environment. Our results show that 1% alginate-0.25% hyaluronic acid microcapsules retain 1.5% alginate physicochemical properties. Moreover, mesenchymal stem cells encapsulated in these hybrid beads show enhanced viability therapeutic protein release and mesenchymal stem cells' potential to differentiate into chondrogenic lineage. Although future studies with additional proteins need to be done in order to approach even more the extracellular matrix features, we have shown that hyaluronic acid protects alginate encapsulated mesenchymal stem cells by providing a niche-like environment and remaining them competent as a sustainable drug delivery system.
- Subjects :
- Alginates pharmacology
Animals
Apoptosis drug effects
Cell Differentiation drug effects
Cell Line
Cell Survival drug effects
Chondrogenesis drug effects
Glucuronic Acid chemistry
Glucuronic Acid pharmacology
Hexuronic Acids chemistry
Hexuronic Acids pharmacology
Hyaluronic Acid pharmacology
Mesenchymal Stem Cells cytology
Mice
Alginates chemistry
Capsules chemistry
Hyaluronic Acid chemistry
Mesenchymal Stem Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1543-8392
- Volume :
- 14
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Molecular pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 28558467
- Full Text :
- https://doi.org/10.1021/acs.molpharmaceut.7b00295