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[THE ROLE OF THE PLANT ARTEMISIA IN SURVIVAL AND INDUCTION OF APOPTOSIS OF B CELLS IN CHRONIC LYMPHOCYTIC LEUKEMIA (CLL)].
- Source :
-
Harefuah [Harefuah] 2017 Feb; Vol. 156 (2), pp. 86-88. - Publication Year :
- 2017
-
Abstract
- Background: The cytotoxic effect of plants such as green tea and turmeric (curcumin) on B chronic lymphocytic leukemia (CLL) cells has been established. The plant Artemisia has been used in China for anti-cancer and anti-malaria applications. In Israel, Artemisia absinthium ("the Chiba") is used to release abdominal pain. In attempts to evaluate the cytotoxic effect of this plant in CLL cells, we prepared a decoction of Artemisia leaves and after filtration used it as an inducer of apoptosis of B CLL cells.<br />Methods: CLL cells were collected from 7 patients in different stages of the disease. Apoptosis was measured using an annexin based flow cytometry assay.<br />Results: First a viability test showed that 100μl/106 cells was the most effective dilution for killing up to 70% cells after 48 hours of incubation. In these conditions Artemisia induced approximately 75% apoptosis in comparison to 32% in the cultures without Artemisia.<br />Conclusions: We concluded that decoction of Artemisia absinthium is a potent inducer of in vitro apoptosis of CLL cells. Our results provide a laboratory basis for further clinical application.
Details
- Language :
- Hebrew
- ISSN :
- 0017-7768
- Volume :
- 156
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Harefuah
- Publication Type :
- Academic Journal
- Accession number :
- 28551901