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Automated Quantification of Early Bone Alterations and Pathological Bone Turnover in Experimental Arthritis by in vivo PET/CT Imaging.
- Source :
-
Scientific reports [Sci Rep] 2017 May 22; Vol. 7 (1), pp. 2217. Date of Electronic Publication: 2017 May 22. - Publication Year :
- 2017
-
Abstract
- The assessment of bone damage is required to evaluate disease severity and treatment efficacy both in arthritis patients and in experimental arthritis models. Today there is still a lack of in vivo methods that enable the quantification of arthritic processes at an early stage of the disease. We performed longitudinal in vivo imaging with [ <superscript>18</superscript> F]-fluoride PET/CT before and after experimental arthritis onset for diseased and control DBA/1 mice and assessed arthritis progression by clinical scoring, tracer uptake studies and bone volume as well as surface roughness measurements. Arthritic animals showed significantly increased tracer uptake in the paws compared to non-diseased controls. Automated CT image analysis revealed increased bone surface roughness already in the earliest stage of the disease. Moreover, we observed clear differences between endosteal and periosteal sites of cortical bone regarding surface roughness. This study shows that in vivo PET/CT imaging is a favorable method to study arthritic processes, enabling the quantification of different aspects of the disease like pathological bone turnover and bone alteration. Especially the evaluation of bone surface roughness is sensitive to early pathological changes and can be applied to study the dynamics of bone erosion at different sites of the bones in an automated fashion.
- Subjects :
- Animals
Arthritis, Experimental etiology
Arthritis, Experimental metabolism
Arthritis, Rheumatoid diagnostic imaging
Arthritis, Rheumatoid etiology
Arthritis, Rheumatoid metabolism
Arthritis, Rheumatoid pathology
Bone and Bones metabolism
Disease Models, Animal
Female
Glucose-6-Phosphatase metabolism
Imaging, Three-Dimensional
Isoenzymes
Mice
Reproducibility of Results
X-Ray Microtomography
Arthritis, Experimental diagnostic imaging
Arthritis, Experimental pathology
Bone and Bones diagnostic imaging
Bone and Bones pathology
Positron Emission Tomography Computed Tomography methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28533505
- Full Text :
- https://doi.org/10.1038/s41598-017-02389-6