Back to Search
Start Over
Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2017 Jun 01; Vol. 53 (45), pp. 6109-6112. - Publication Year :
- 2017
-
Abstract
- We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb <superscript>3+</superscript> ] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.
- Subjects :
- Aminopyridines pharmacology
Benzimidazoles pharmacology
Binding Sites
Biosensing Techniques methods
Cell Line, Tumor
Coordination Complexes chemical synthesis
Cyclin-Dependent Kinase 4 antagonists & inhibitors
Cyclin-Dependent Kinase 4 metabolism
Fluorescence
Fluorescent Dyes chemical synthesis
Fluorescent Dyes chemistry
Heterocyclic Compounds, 1-Ring chemical synthesis
Humans
Peptides chemical synthesis
Peptides chemistry
Phosphorylation
Purines pharmacology
Roscovitine
Terbium chemistry
Tryptophan chemistry
Coordination Complexes chemistry
Cyclin-Dependent Kinase 4 analysis
Fluorescent Dyes metabolism
Heterocyclic Compounds, 1-Ring chemistry
Peptides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 53
- Issue :
- 45
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 28530267
- Full Text :
- https://doi.org/10.1039/c6cc09948c