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Determination of fat-soluble vitamins in a pharmaceutical dosage form by solid-phase extraction and reversed-phase liquid chromatography.

Authors :
Savolainen KE
Pynnönen KM
Lapinjoki SP
Vidgren MT
Source :
Journal of pharmaceutical sciences [J Pharm Sci] 1988 Sep; Vol. 77 (9), pp. 802-3.
Publication Year :
1988

Abstract

A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid chromatography (HPLC). Cholecalciferol, alpha-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns. The vitamins were then chromatographed on a Hypersil 5-microns C18 column, using a water:methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7-2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes.

Details

Language :
English
ISSN :
0022-3549
Volume :
77
Issue :
9
Database :
MEDLINE
Journal :
Journal of pharmaceutical sciences
Publication Type :
Academic Journal
Accession number :
2852248
Full Text :
https://doi.org/10.1002/jps.2600770918