Back to Search
Start Over
Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells.
Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells.
- Source :
-
Journal of endocrinological investigation [J Endocrinol Invest] 2017 Oct; Vol. 40 (10), pp. 1133-1143. Date of Electronic Publication: 2017 May 15. - Publication Year :
- 2017
-
Abstract
- Purpose: Testosterone by promoting different metabolic pathways contributes to short-term homeostasis of skeletal muscle, the largest insulin-sensitive tissue and the primary site for insulin-stimulated glucose utilization. Despite evidences indicate a close relationship between testosterone and glucose metabolism, the molecular mechanisms responsible for a possible testosterone-mediated insulin-like effects on skeletal muscle are still unknown.<br />Methods: Here we used undifferentiated proliferating or differentiated human fetal skeletal muscle cells (Hfsmc) to investigate the short-term effects of testosterone on the insulin-mediated biomolecular metabolic machinery. GLUT4 cell expression, localization and the phosphorylation/activation of AKT, ERK, mTOR and GSK3β insulin-related pathways at different time points after treatment with testosterone were analyzed.<br />Results: Independently from cells differentiation status, testosterone, with an insulin-like effect, induced Glut4-mRNA expression, GLUT4 protein translocation to the cytoplasmic membrane, while no effect was observed on GLUT4 protein expression levels. Furthermore, testosterone treatment modulated the insulin-dependent signal transduction pathways inducing a rapid and persistent activation of AKT, ERK and mTOR, and a transient inhibition of GSK3β. T-related effects were shown to be androgen receptor dependent.<br />Conclusion: All together our data indicate that testosterone through the activation of non-genomic pathways, participates in skeletal muscle glucose metabolism by inducing insulin-related effects.
- Subjects :
- Androgens pharmacology
Cells, Cultured
Fetus drug effects
Humans
Hypoglycemic Agents pharmacology
In Vitro Techniques
Insulin Resistance
Male
Muscle, Skeletal cytology
Muscle, Skeletal drug effects
Phosphorylation drug effects
Biomarkers metabolism
Fetus metabolism
Insulin pharmacology
Muscle, Skeletal metabolism
Signal Transduction drug effects
Testosterone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1720-8386
- Volume :
- 40
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of endocrinological investigation
- Publication Type :
- Academic Journal
- Accession number :
- 28508346
- Full Text :
- https://doi.org/10.1007/s40618-017-0686-y