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Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells.

Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells.

Authors :
Antinozzi C
Marampon F
Corinaldesi C
Vicini E
Sgrò P
Vannelli GB
Lenzi A
Crescioli C
Di Luigi L
Source :
Journal of endocrinological investigation [J Endocrinol Invest] 2017 Oct; Vol. 40 (10), pp. 1133-1143. Date of Electronic Publication: 2017 May 15.
Publication Year :
2017

Abstract

Purpose: Testosterone by promoting different metabolic pathways contributes to short-term homeostasis of skeletal muscle, the largest insulin-sensitive tissue and the primary site for insulin-stimulated glucose utilization. Despite evidences indicate a close relationship between testosterone and glucose metabolism, the molecular mechanisms responsible for a possible testosterone-mediated insulin-like effects on skeletal muscle are still unknown.<br />Methods: Here we used undifferentiated proliferating or differentiated human fetal skeletal muscle cells (Hfsmc) to investigate the short-term effects of testosterone on the insulin-mediated biomolecular metabolic machinery. GLUT4 cell expression, localization and the phosphorylation/activation of AKT, ERK, mTOR and GSK3β insulin-related pathways at different time points after treatment with testosterone were analyzed.<br />Results: Independently from cells differentiation status, testosterone, with an insulin-like effect, induced Glut4-mRNA expression, GLUT4 protein translocation to the cytoplasmic membrane, while no effect was observed on GLUT4 protein expression levels. Furthermore, testosterone treatment modulated the insulin-dependent signal transduction pathways inducing a rapid and persistent activation of AKT, ERK and mTOR, and a transient inhibition of GSK3β. T-related effects were shown to be androgen receptor dependent.<br />Conclusion: All together our data indicate that testosterone through the activation of non-genomic pathways, participates in skeletal muscle glucose metabolism by inducing insulin-related effects.

Details

Language :
English
ISSN :
1720-8386
Volume :
40
Issue :
10
Database :
MEDLINE
Journal :
Journal of endocrinological investigation
Publication Type :
Academic Journal
Accession number :
28508346
Full Text :
https://doi.org/10.1007/s40618-017-0686-y