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Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway.
- Source :
-
Life sciences [Life Sci] 2017 Jul 01; Vol. 180, pp. 36-41. Date of Electronic Publication: 2017 May 10. - Publication Year :
- 2017
-
Abstract
- Aims: Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae flavescens, has been recently found to be beneficial in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, mainly through its anti-inflammatory effect. In the present study, we tested the effect of MAT on ongoing EAE and defined possible mechanisms underlying its effects on myelination and oligodendrocytes.<br />Main Methods: EAE was induced in C57BL/6 mice and MAT treatment was started at disease onset. Clinical scores were monitored daily; spinal cords and the corpus callosum brain region of mice were harvested on day 23 p.i. for inflammatory infiltration and demyelination of the central nervous system. Myelin content and the development of oligodendrocytes and their precursors were determined by immunostaining, and expression of p-Akt, p-mTOR, p-PI3K, and p-P70S6 was determined by Western blot.<br />Key Findings: MAT effectively suppressed EAE severity and increased the expression of proteolipid protein, a myelin protein that is a marker of CNS myelin. MAT treatment largely increased the number of mature oligodendrocytes, and significantly activated the PI3K/Akt/mTOR signaling pathway, which is required for oligodendrocyte survival and axon myelination.<br />Significance: These findings demonstrate a beneficial effect of MAT on oligodendrocyte differentiation and myelination during EAE, most likely through activating the PI3K/Akt/mTOR signaling pathway.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Alkaloids isolation & purification
Animals
Autoimmunity drug effects
Cell Differentiation drug effects
Corpus Callosum drug effects
Corpus Callosum pathology
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental pathology
Female
Mice
Mice, Inbred C57BL
Multiple Sclerosis pathology
Myelin Sheath metabolism
Oligodendroglia metabolism
Phosphatidylinositol 3-Kinase metabolism
Proto-Oncogene Proteins c-akt metabolism
Quinolizines isolation & purification
Severity of Illness Index
Signal Transduction drug effects
Sophora chemistry
TOR Serine-Threonine Kinases metabolism
Matrines
Alkaloids pharmacology
Encephalomyelitis, Autoimmune, Experimental drug therapy
Multiple Sclerosis drug therapy
Oligodendroglia drug effects
Quinolizines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 180
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28499934
- Full Text :
- https://doi.org/10.1016/j.lfs.2017.05.010