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Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment.
- Source :
-
Scientific reports [Sci Rep] 2017 May 10; Vol. 7 (1), pp. 1667. Date of Electronic Publication: 2017 May 10. - Publication Year :
- 2017
-
Abstract
- Orchestration of bone repair processes requires crosstalk between different cell populations, including immune cells and mesenchymal stem/stromal cells (MSC). Extracellular vesicles (EV) as mediators of these interactions remain vastly unexplored. Here, we aimed to determine the mechanism of MSC recruitment by Dendritic Cells (DC), hypothesising that it would be mediated by EV. Primary human DC-secreted EV (DC-EV), isolated by ultracentrifugation, were characterized for their size, morphology and protein markers, indicating an enrichment in exosomes. DC-EV were readily internalized by human bone marrow-derived MSC, without impacting significantly their proliferation or influencing their osteogenic/chondrogenic differentiation. Importantly, DC-EV significantly and dose-dependently promoted MSC recruitment across a transwell system and enhanced MSC migration in a microfluidic chemotaxis assay. DC-EV content was analysed by chemokine array, indicating the presence of chemotactic mediators. Osteopontin and matrix metalloproteinase-9 were confirmed inside EV. In summary, DC-EV are naturally loaded with chemoattractants and can contribute to cell recruitment, thus inspiring the development of new tissue regeneration strategies.
- Subjects :
- Cell Differentiation
Cell Movement
Cell Proliferation
Cells, Cultured
Dendritic Cells ultrastructure
Endocytosis
Exosomes metabolism
Exosomes ultrastructure
Extracellular Vesicles ultrastructure
Humans
Dendritic Cells metabolism
Extracellular Vesicles metabolism
Mesenchymal Stem Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28490808
- Full Text :
- https://doi.org/10.1038/s41598-017-01809-x