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Systemic and mucosal immune responses elicited by intranasal immunization with a pneumococcal bacterium-like particle-based vaccine displaying pneumolysin mutant Plym2.
- Source :
-
Immunology letters [Immunol Lett] 2017 Jul; Vol. 187, pp. 41-46. Date of Electronic Publication: 2017 May 06. - Publication Year :
- 2017
-
Abstract
- Pneumolysin (Ply) is an important virulence factor in pneumococcal infection and a conserved cholesterol-binding cytotoxin expressed by all serotypes of Streptococcus pneumoniae. We previously developed a highly detoxified Ply mutant designated Plym2 by replacement of two amino acids (C428G and W433F), which lost cytotoxicity but retained the ability to induce neutralizing antibodies. In the present work, we applied bacterium-like particles (BLPs) as a carrier and immunostimulant for the development of a Plym2 intranasal vaccine, in which the Plym2 protein was displayed on the surface of BLPs. Intranasal immunization of mice with BLP-Plym2 not only induced a high level of serum IgG antibodies but also a high level of mucosal SIgA antibodies in lung lavages. Antiserum induced by the BLP-Plym2 vaccine elicited high-titer neutralization activity which could inhibit the hemolysis of wild-type Ply. In conclusion, the BLP-Plym2 vaccine was demonstrated to be a promising strategy for intranasal immunization to enhance both systemic and mucosal immune responses.<br /> (Copyright © 2017 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Intranasal
Amino Acid Substitution
Animals
Antibodies, Bacterial immunology
Bacterial Proteins genetics
Bacterial Proteins immunology
Female
Mice
Mice, Inbred BALB C
Streptococcus pneumoniae genetics
Streptococcus pneumoniae immunology
Immunity, Mucosal drug effects
Immunization
Immunoglobulin A immunology
Mutation, Missense
Pneumococcal Vaccines genetics
Pneumococcal Vaccines immunology
Pneumococcal Vaccines pharmacology
Streptolysins genetics
Streptolysins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0542
- Volume :
- 187
- Database :
- MEDLINE
- Journal :
- Immunology letters
- Publication Type :
- Academic Journal
- Accession number :
- 28487097
- Full Text :
- https://doi.org/10.1016/j.imlet.2017.05.003