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Mussel-Inspired Surface-Imprinted Sensors for Potentiometric Label-Free Detection of Biological Species.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2017 Jun 06; Vol. 56 (24), pp. 6833-6837. Date of Electronic Publication: 2017 May 09. - Publication Year :
- 2017
-
Abstract
- Using sensors to quantify clinically relevant biological species has emerged as a fascinating research field due to their potential to revolutionize clinical diagnosis and therapeutic monitoring. Taking advantage of the wide utility in clinical analysis and low cost of potentiometric ion sensors, we demonstrate a method to use such ion sensors to quantify bioanalytes without chemical labels. This is achieved by combination of chronopotentiometry with a mussel-inspired surface imprinting technique. The biomimetic sensing method is based on a blocking mechanism by which the recognition reaction between the surface imprinted polymer and a bioanalyte can block the current-induced ion transfer of an indicator ion, thus causing a potential change. The present method offers high sensitivity and excellent selectivity for detection of biological analytes. As models, trypsin and yeast cells can be measured at levels down to 0.03 U mL <superscript>-1</superscript> and 50 CFU mL <superscript>-1</superscript> , respectively.<br /> (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Animals
Escherichia coli isolation & purification
Horseradish Peroxidase analysis
Limit of Detection
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Molecular Imprinting
Serum Albumin, Bovine analysis
Staphylococcus aureus isolation & purification
Surface Properties
Thrombin analysis
Trypsin analysis
Vibrio alginolyticus isolation & purification
Yeasts ultrastructure
Biosensing Techniques instrumentation
Bivalvia
Potentiometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 56
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 28485518
- Full Text :
- https://doi.org/10.1002/anie.201701892