A novel label-free electrochemical immunosensor based on the enhanced catalytic currents of oxygen reduction by AuAg hollow nanocrystals for detecting carbohydrate antigen 199.

Authors :: Wang R
Feng JJ
Liu WD
Jiang LY
Wang AJ
Source :: Biosensors & bioelectronics [Biosens Bioelectron] 2017 Oct 15; Vol. 96, pp. 152-158. Date of Electronic Publication: 2017 May 04.
Publication Year :: 2017
Abstract: Herein, bimetallic alloyed AuAg hollow nanocrystals (AuAg HNCs) were prepared by a simple one-pot aqueous method using polycytidysic acid (PCA) as the green growth-directing agent. The novel immunosensor for carbohydrate antigen 199 (CA199) was further constructed based on the enhanced catalytic currents of oxygen reduction reaction (ORR) by AuAg HNCs. By virtue of the good biocompatibility and catalytic activity of AuAg HNCs, the immunosensor exhibited superior analytical performance for the assay of CA199 under the optimal experimental conditions, the ORR signals linearly decreased with the increased CA199 concentrations in the range of 1 ~ 30UmL <superscript>-1</superscript> , with the low detection limit of 0.228UmL <superscript>-1</superscript> , improved stability, reproducibility and selectivity.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)

Subjects :: Antibodies, Immobilized chemistry
Antigens, Tumor-Associated, Carbohydrate analysis
Catalysis
Humans
Immunoassay methods
Limit of Detection
Metal Nanoparticles ultrastructure
Oxidation-Reduction
Antigens, Tumor-Associated, Carbohydrate blood
Biosensing Techniques methods
Electrochemical Techniques methods
Gold chemistry
Metal Nanoparticles chemistry
Oxygen chemistry
Silver chemistry

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