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PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.
- Source :
-
PLoS genetics [PLoS Genet] 2017 May 08; Vol. 13 (5), pp. e1006789. Date of Electronic Publication: 2017 May 08 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase δ with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling.
- Subjects :
- Cell Line, Tumor
Chromatin genetics
Chromatin metabolism
DNA-Directed DNA Polymerase genetics
DNA-Directed DNA Polymerase metabolism
Humans
Proliferating Cell Nuclear Antigen genetics
Schizosaccharomyces metabolism
DNA Replication
Proliferating Cell Nuclear Antigen metabolism
Schizosaccharomyces genetics
Ubiquitination
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 13
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 28481910
- Full Text :
- https://doi.org/10.1371/journal.pgen.1006789