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Development of a new model of reconstituted mouse epidermis and characterization of its response to proinflammatory cytokines.
- Source :
-
Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2018 Feb; Vol. 12 (2), pp. e1098-e1107. Date of Electronic Publication: 2017 Jun 26. - Publication Year :
- 2018
-
Abstract
- The development of three-dimensional models of reconstituted mouse epidermis (RME) has been hampered by the difficulty to maintain murine primary keratinocyte cultures and to achieve a complete epidermal stratification. In this study, a new protocol is proposed for the rapid and convenient generation of RME, which reproduces accurately the architecture of a normal mouse epidermis. During RME morphogenesis, the expression of differentiation markers such as keratins, loricrin, filaggrin, E-cadherin and connexins was followed, showing that RME structure at day 5 was similar to those of a normal mouse epidermis, with the acquisition of the natural barrier function. It was also demonstrated that RME responded to skin-relevant proinflammatory cytokines by increasing the expression of antimicrobial peptides and chemokines, and inhibiting epidermal differentiation markers, as in the human system. This new model of RME is therefore suitable to investigate mouse epidermis physiology further and opens new perspectives to generate reconstituted epidermis from transgenic mice.<br /> (Copyright © 2017 John Wiley & Sons, Ltd.)
- Subjects :
- Adherens Junctions drug effects
Adherens Junctions metabolism
Animals
Animals, Newborn
Biomarkers metabolism
Cell Differentiation drug effects
Filaggrin Proteins
Gap Junctions drug effects
Gap Junctions metabolism
Mice, Inbred C57BL
Morphogenesis drug effects
Receptors, Cytokine metabolism
Cytokines toxicity
Epidermis drug effects
Inflammation Mediators toxicity
Models, Biological
Subjects
Details
- Language :
- English
- ISSN :
- 1932-7005
- Volume :
- 12
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of tissue engineering and regenerative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 28477582
- Full Text :
- https://doi.org/10.1002/term.2442